Iso-Seq

note

Identifies transcripts in PacBio single-molecule sequencing data (HiFi reads).

https://github.com/PacificBiosciences/IsoSeq

Supports outputs generated by two commands:

• IsoSeq refine trims poly(A) tails and removes concatemers.

• IsoSeq cluster performs clustering using hierarchical n*log(n) alignment and iterative cluster merging.

File search patterns

isoseq/cluster-csv:
  contents: cluster_id
  fn: "*cluster_report.csv"
  num_lines: 1
isoseq/refine-csv:
  contents: id,strand,fivelen,threelen,polyAlen,insertlen,primer
  fn: "*.csv"
isoseq/refine-json:
  contents: '"num_reads_fl"'
  fn: "*.json"